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Fasciculus:Summary of SAGE.svg

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E Vicipaedia

Sua resolutio ‎(fasciculus SVG, nominale 938 × 756 elementa imaginalia, magnitudo fasciculi: 180 chiliocteti)

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Summarium

Descriptio
English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In SAGE, the ds-cDNA is digested by restriction enzymes (at location ‘X’ and ‘X’+11) to produce 11-nucleotide ‘digitag’ fragments. These digitags are concatenated and sequences using long-read sanger sequencing (different shades of blue indicate digitags from different genes). The sequences are deconvoluted to find the occurrence number of each digitag. Digitag can be used to report on transcription of the gene that the digitag came from is known.[1]
Datum
Fons Opus proprium
Auctor Thomas Shafee
Other versions

Also in series:

  • RNA Microarray
    RNA Microarray
  • RNA-Seq
    RNA-Seq
  • SAGE
    SAGE

Potestas usoris

I, the copyright holder of this work, hereby publish it under the following license:
w:en:Creative Commons
attributio
This file is licensed under the Creative Commons Attribution 4.0 International license.
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  • communicare – copiare, distribuere et committere hoc opus
  • to remix – to adapt the work
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  • attributio – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  1. ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.

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depicts Anglica

Serial analysis of gene expression Anglica

copyright status Anglica

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inceptio

5 Octobris 2016

Historia fasciculi

Presso die vel tempore fasciculum videbis, sicut tunc temporis apparuit.

Dies/TempusMinutioDimensionesUsorSententia
recentissima12:21, 13 Februarii 2017Minutum speculum redactionis 12:21, 13 Februarii 2017 factae938 × 756 (180 chiliocteti)Evolution and evolvabilitytext as paths to avoid artefacts
10:48, 13 Februarii 2017Minutum speculum redactionis 10:48, 13 Februarii 2017 factae938 × 756 (104 chiliocteti)Evolution and evolvabilityupdate sequence text to 'sans-serif' open font for more robust rendering
05:27, 7 Octobris 2016Minutum speculum redactionis 05:27, 7 Octobris 2016 factae938 × 756 (104 chiliocteti)Evolution and evolvabilityce
05:23, 7 Octobris 2016Minutum speculum redactionis 05:23, 7 Octobris 2016 factae938 × 756 (102 chiliocteti)Evolution and evolvabilityce
05:16, 7 Octobris 2016Minutum speculum redactionis 05:16, 7 Octobris 2016 factae938 × 756 (104 chiliocteti)Evolution and evolvabilitydigitag-->tag
05:08, 5 Octobris 2016Minutum speculum redactionis 05:08, 5 Octobris 2016 factae1 020 × 821 (106 chiliocteti)Evolution and evolvabilityUser created page with UploadWizard

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